Dataset: Acropora hyacinthus ITS2 amplicon sequencing

Name: ITS2 amplicon sequences from Acropora hyacinthus samples collected at mutliple timepoints in Moorea, French Polynesia after the mass bleaching event of 2019
Published: 2022-06-29
Keywords: coral bleaching, Acropora hyacinthus, Moorea, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.876564.1

Spatial Extent: N:-14.4751 E:-149.817 S:-17.4731 W:-149.818

Temporal Extent: 2019-05-19 - 2019-10-09

Project:

RAPID: Collaborative Research: Studies of recovery from bleaching in Acropora hyacinthus: epigenetic shifts, impacts on reproductive biology and carry-over effects (Moorea coral bleaching)

Principal Investigator:

Marie Strader (Auburn University)

Co-Principal Investigator:

Gretchen E. Hofmann (University of California-Santa Barbara, UCSB-MSI)

Contact:

Marie Strader (Auburn University)

BCO-DMO Data Manager:

Taylor Heyl (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2022-06-29

Restricted:

Release Date:

2022-12-31

Validated:

Yes

Current State:

Final no updates expected

ITS2 amplicon sequences from Acropora hyacinthus samples collected at mutliple timepoints in Moorea, French Polynesia after the mass bleaching event of 2019

Abstract:

This dataset represents ITS2 amplicon sequences from Acropora hyacinthus samples collected at mutliple timepoints in Moorea, French Polynesia after the mass bleaching event of 2019.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2022-06-29	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.876564.1	2022-08-09

Methods & Sampling

To assess in hospite symbiont community dynamics in a major reef-building coral, Acropora hyacinthus, during a mass bleaching event we sampled small pieces of tissue from each colony and performed ITS2 amplicon sequencing. Samples were collected at the height of the bleaching event (May 2019), 3 months after (August 2019) and 5 months later (October 2019). Samples were collected at 3 different reef zones, backreef (~1 meter depth), shallow forereef (~5 meter depth), and deeper forereef (~14 meter depth).

Raw sequence data have been deposited in the NCBI Sequence Read Archive (SRA) under BioProject number PRJNA833391. This dataset also includes relevant metadata for each sample.

Data Processing Description

BCO-DMO Processing Description:
- Converted date columns to format YYYY-MM-DD
- Adjusted field/parameter names to comply with BCO-DMO naming conventions
- Converted latitude and longitude from South and West to North and East and split columns
- Added a conventional header with dataset name, PI names, version date

Data Files

File
amplicon_sequencing.csv (Comma Separated Values (.csv), 22.65 KB) MD5:60c88de32f57df18e82fd80974767bec Primary data file for dataset ID 876564

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1935308
NSF Division of Ocean Sciences	OCE-1935305

Deployments

None available yet

Instruments

None available yet

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Accession	NCBI accession number	unitless	accession_number
Sample_Name	name of individual sample	unitless	sample
BioSample	NCBI BioSample number	unitless	sample
Details	description of colony and location of coral reef	unitless	comment
Host	species identification of coral host	unitless	species
Location	location of sample site	unitless	site
Date	date of sample collection in format YYYY-MM-DD Format: %Y-%m-%d	unitless	date
Depth	water depth of sample site	meters (m)	depth
Latitude	latitude North of sample site	decimal degrees	lat
Longitude	longitude East of sample site	decimal degrees	lon

Database

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Dataset: Acropora hyacinthus ITS2 amplicon sequencing