Dataset: Crustose coralline algae calcification experiment data

Name: Clathromorphum compactum and C. nereostratum calcification experiment data involving multiple temperatures and pCO2 levels (CorallineAlgaePaleo-pH)
Published: 2022-04-04
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.871633.1

Temporal Extent: 2015-10-08 - 2016-02-09

Project:

Collaborative Research: Development and application of a method using coralline algae to reconstruct past changes in pH and impacts on calcification (CorallineAlgaePaleo-pH)

Principal Investigator:

Justin B. Ries (Northeastern University)

Co-Principal Investigator:

Douglas B. Rasher (Bigelow Laboratory for Ocean Sciences)

Branwen Williams (University of California-San Diego, UCSD-SIO)

Scientist:

Isaac Westfield (Northeastern University)

Contact:

Justin B. Ries (Northeastern University)

BCO-DMO Data Manager:

Sawyer Newman (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2022-04-04

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Clathromorphum compactum and C. nereostratum calcification experiment data involving multiple temperatures and pCO2 levels (CorallineAlgaePaleo-pH)

Abstract:

Experimental calcification results from crustose coralline algae experiment involving multiple temperatures and pCO2 levels. Clathromorphum compactum and C. nereostratum were used for this experiment.

Expand/Collapse All

Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2022-04-04	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.871633.1	2022-04-19

Methods & Sampling

Methodology:

Sampling and analytical procedures:

C. compactum and C. nereostratum cultured for 4 months under 12 pCO2/T treatments in 42 liter aquaria.

Seawater samples were obtained every ~14 days using 250 mL ground-glass-stoppered borosilicate glass bottles for measurement of total alkalinity (TA) and dissolved inorganic carbon (DIC) and other carbonate parameters.

Temp, salinity, pH were measured three times weekly.

For buoyant weight, specimens were suspended beneath a balance in an aquarium at 4 cm depth in seawater of constant temperature and salinity.

Specimens were dosed with calcein before the experiment and the vertical growth was determined by measuring the vertical growth from the resulting calcein line to the surface.

Data Processing Description

Researcher processing notes:

R Project and Microsoft Excel were used for all calculations and figure creation.
CO2Sys used to calculate all carbonate parameters.

Data Files

File
final_data_file_westfield_updated_04-14-2022-1.csv (Comma Separated Values (.csv), 62.65 KB) MD5:e6bdcf9f31599dbcab0e7257b52aa4f7 Primary data file for dataset ID 871633

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1459706
NSF Division of Ocean Sciences	OCE-1459827
NSF Arctic Sciences	PLR-1316141

Deployments

None available yet

Instruments

MARIANDA VINDTA 3C total inorganic carbon and titration alkalinity analyser

Supplied Name: VINDTA 3C (Marianda Corporation, Kiel, Germany)

Supplied Description:

Measures total alkalinity and DIC using closed-cell potentiometric Gran titration and coulometry (UIC 5400), with both methods calibrated using certified Dickson DIC/TA standards.

Instrument Type

Generic Name: MARIANDA VINDTA 3C total inorganic carbon and titration alkalinity analyser

Acronym: inorganic carbon and alkalinity analyser

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0481/

Generic Description:

The Versatile INstrument for the Determination of Total inorganic carbon and titration Alkalinity (VINDTA) 3C is a laboratory alkalinity titration system combined with an extraction unit for coulometric titration, which simultaneously determines the alkalinity and dissolved inorganic carbon content of a sample. The sample transport is performed with peristaltic pumps and acid is added to the sample using a membrane pump. No pressurizing system is required and only one gas supply (nitrogen or dry and CO2-free air) is necessary. The system uses a Metrohm Titrino 719S, an ORION-Ross pH electrode and a Metrohm reference electrode. The burette, the pipette and the analysis cell have a water jacket around them. Precision is typically +/- 1 umol/kg for TA and/or DIC in open ocean water.

scale

Supplied Name: Nimbus NBL 423e Precision Balance (±0.0002 precision, ±0.002 accuracy; AE Adam®; Oxford, Connecticut, USA)

Supplied Description:

Used for all mass measurements.

Instrument Type

Generic Name: scale

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/

Generic Description:

An instrument used to measure weight or mass.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Sample_ID	Individual IDs for each specimen	unitless	sample
Species	Species name of specimen	unitless	species
Start_Date	Date of the start of experiment for that specimen; YYYY-MM-DD Format: %Y-%m-%d	unitless	start_date
Finish_Date	Date of the end of the experiment for that specimen; YYYY-MM-DD Format: %Y-%m-%d	unitless	end_date
Days_Elapsed	Total number of days in experiment for that specimen	days	duration
Mean_pCO2_uatm	Mean pCO2 of experimental tank	ppm	pCO2
Mean_Temp_c	Mean temperature of experimental tank	Celsius	temp
Mean_pH	Mean pH of experimental tank	unitless	pH
Tank	Tank number within a temperature/pCO2 combination	unitless	tank
Initial_Buoyant_Weight_g	Initial buoyant weight of specimen	grams	no_bcodmo_term
Final_Buoyant_Weight_g	Final buoyant weight of specimen	grams	no_bcodmo_term
Surface_Area_cm2	Final surface area of specimen	cm2	surface_area
Vertical_Extension_cm	Vertical growth of that specimen	cm2	growth
Calcification_Rate_mg_per_cm2_year	Mass of calcification per surface area projected for a year	mg/cm2/year	calcification

Database

Contribute Data

Dataset: Crustose coralline algae calcification experiment data