Dataset: P. homomalla development (fine)

Name: Cell counts for 11 P. homomalla embryos taken every 5-10 minutes from fertilization to 13 hours after fertilization from adult corals collected in Round Bay, St. John, USVI in July of 2019
Published: 2021-07-15
Keywords: cleavage polyembryony, Cnidaria, ex situ rearing, Octocorallia, Plexauridae, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.855801.1

Spatial Extent: N:18.345 E:-64.681 S:18.345 W:-64.681

Temporal Extent: 2019-07-21

Project:

Collaborative Research: Pattern and process in the abundance and recruitment of Caribbean octocorals (Octocoral Community Dynamics)

Principal Investigator:

Howard Lasker (State University of New York at Buffalo, SUNY Buffalo)

Scientist:

Kaitlyn Tonra (State University of New York at Buffalo, SUNY Buffalo)

Christopher Wells (State University of New York at Buffalo, SUNY Buffalo)

Contact:

Kaitlyn Tonra (State University of New York at Buffalo, SUNY Buffalo)

Technician:

Emily Anderson (State University of New York at Buffalo, SUNY Buffalo)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2021-07-15

Restricted:

Release Date:

2021-09-01

Validated:

Yes

Current State:

Final no updates expected

Cell counts for 11 P. homomalla embryos taken every 5-10 minutes from fertilization to 13 hours after fertilization from adult corals collected in Round Bay, St. John, USVI in July of 2019

Abstract:

Cell counts for 11 Plexaura homomalla embryos taken every 5-10 minutes from fertilization to 13 hours after fertilization. Adult corals used in this study were collected in Round Bay, St. John, USVI on July 14–15, 2019. The results publication for this dataset is Tonra et al. (2021, see Fig. 3).

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2021-07-15	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.855801.1	2021-09-14

Methods & Sampling

Methodology:

Eleven female and four male branches of Plexaura homomalla were collected from adult colonies on an octocoral-dominated reef in Round Bay, St. John, USVI on July 14–15, 2019. All branches were about 15 cm long. Colonies were determined to be gravid in the field by cutting a 5-cm piece diagonally and looking for spermaries or eggs. Colonies were transported to the Virgin Islands Environmental Research Station and maintained in a sea table.

We monitored for signs of spawning for 5 hr starting at sunset starting one night after the full moon from July 17–23, 2019. Eggs and sperm were collected together with 60- mL syringes as they were released from the colonies and were stored in water from the tank for 30 min to ensure fertilization. We refer to the time at which gametes were collected as t = 0. On the third night of spawning detailed observations of embryo development were conducted by counting the number of cells in 11 embryos in triplicate every 10 min for the first 2 hr after fertilization, every 5 min for the next 4 hr, and every hour for the next 8 hr. Individual embryos were kept in separate containers. We stopped counting the number of cells after embryos reached 128 cells because further divisions became difficult to detect.

Instruments:
dissecting microscope, hand lens

Data Processing Description

BCO-DMO data manager processing notes:

* Data from file NSF.Phomomalla_development_fine_monitoring.xlsx sheet "data" imported into the BCO-DMO data system.
* Added column ISO_DateTime_UTC_Start from times and dates provided in local time UTC-4.
* Renamed data columns to meet BCO-DMO naming conventions (only [a-zA-Z0-9] and underscores). Periods changed to underscores.
* Renamed data columns to meet BCO-DMO naming conventions (only [a-zA-Z0-9] and underscores). Periods changed to underscores.

Data Files

File
p_homomalla_dev_fine.csv (Comma Separated Values (.csv), 3.96 KB) MD5:09d184919184e699e90a94f7aba96bdf Primary data file for dataset ID 855801

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1756381

Deployments

None available yet

Instruments

None available yet

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
start_time	start time of measurement	unitless	time_local
time	time relative to the time eggs were collected	unitless	time_elapsed
date	date of observation Format: %m-%d-%y	unitless	date_local
indiv_01	number of cells composing individual 01 (can be 1 to 64, 128 [more than 64], or X [dead])	unitless	count
indiv_02	number of cells composing individual 02 (can be 1 to 64, 128 [more than 64], or X [dead])	unitless	count
indiv_03	number of cells composing individual 03 (can be 1 to 64, 128 [more than 64], or X [dead])	unitless	count
indiv_04	number of cells composing individual 04 (can be 1 to 64, 128 [more than 64], or X [dead])	unitless	count
indiv_05	number of cells composing individual 05 (can be 1 to 64, 128 [more than 64], or X [dead])	unitless	count
indiv_06	number of cells composing individual 06 (can be 1 to 64, 128 [more than 64], or X [dead])	unitless	count
indiv_07	number of cells composing individual 07 (can be 1 to 64, 128 [more than 64], or X [dead])	unitless	count
indiv_08	number of cells composing individual 08 (can be 1 to 64, 128 [more than 64], or X [dead])	unitless	count
indiv_09	number of cells composing individual 09 (can be 1 to 64, 128 [more than 64], or X [dead])	unitless	count
indiv_10	number of cells composing individual 10 (can be 1 to 64, 128 [more than 64], or X [dead])	unitless	count
indiv_11	number of cells composing individual 11 (can be 1 to 64, 128 [more than 64], or X [dead])	unitless	count
ISO_DateTime_UTC_Start	start Datetime of that counting period in ISO 8601 format yyyy-mm-ddTHH:MMZ (UTC) Format: %Y-%m-%dT%H:%MZ	unitless	ISO_DateTime_UTC

Database

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Dataset: P. homomalla development (fine)